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PLANT PIGMENTS. http://members.aol.com/diaspore/vhouse/biology/projects/photosynthesis/woida/meaning_of_light.html#question_1
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CHROMATOGRAPHY USE PENCIL not pen, to mark starting line !!!!!
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ADD PLANT PIGMENTS
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PLACE IN JAR Image by Riedell IMPORTANT: Make sure tip of paper is touching solvent Make sure pigment line is ABOVE solvent Put lid on jar !!!!!!!!
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Important http://www.phschool.com/science/biology_place/labbench/lab4/pigsep.html Don’t let solvent front run off end of paper !
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Marker Chromatography
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R f = Distance pigment migrated Distance solvent front migrated
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PLANT PIGMENTS Carotene is orange yellow Xanthophyll is lemon yellow Chlorophyll a is blue green Chlorophyll b is yellow green.
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Factors that affect movement of molecules during chromatography 1.___________of molecule in solvent used (better it dissolves… farther it will move) Solubility SOLVENT used: Chlorophyll: 9:1 Petroleum ether : acetone Markers: water
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Factors that affect movement of molecules during chromatography 2. _________of molecule (bigger molecules move slower) Size http://elvis.phys.lsu.edu/svoboda/post/figs/SumoWrestler.BMP
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Factors that affect movement of molecules during chromatography 3. _______________________ used (EX: Attraction of molecules to paper) Attraction to media http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-1/ch5_mol-seive.jpg http://www.cofc.edu/~kinard/Applets/GasChromatographFID.gif Lots of different kinds of chromatography
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Factors that affect movement of molecules during chromatography 4. __________allowed to run Time http://www1.istockphoto.com/file_thumbview_approve/581259/2/istockphoto_581259_big_time.jpg Longer time = More separation
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CHLOROPHYLL FLUORESCENCE
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