1. Compound light microscope

2. A Compound Light Microscope  Allows us to view specimens that are too small to be seen with the naked eye  The specimen must be thin enough for light to pass through

3. How the microscope works 1.Light from the source passes through the diaphragm, the stage, and then through the specimen 2.The light passes through the objective lens 3.The enlarged image (picture) is produced 4.The image is then magnified again by the ocular or eye piece lens 5.The final image you see is enlarged (made bigger), upside down and backwards

4. The letter P through a microscope

5. Using the Microscope TO BEGIN:  Always carry the microscope by the arm and support the base  Turn on light  The specimen slide should have a cover slip.  Place it on the stage and secure with the stage clips  Start on LOW POWER with the stage in the lowest position  Use the coarse adjustment to bring the specimen into view, use the fine adjustment to make the specimen clear

6. To switch objectives:  Once you are focused, you should center the structure that you want to see then move the NOSEPIECE to medium power.  Use the coarse and fine adjustment to bring the specimen into clear view.  Once you have focused and centered in medium power, use the NOSEPIECE to move to high power.  You should only use the fine adjustment to focus at high power  Never use coarse adjustment on high power NEVER-EVER-EVER-EVER-EVER-EVER…

7. When you’re finished with the microscope:  Change the objective to the lowest power.  Lower the stage all the way down using the coarse adjustment.  Turn off the light source, unplug the cord, and coil the cord around the base.  Carefully carry (grasping the arm and supporting the base) back to the counter near the storage cabinet.  Follow the directions of your instructor.