1. LEC Biopsy Tool Group Members: Hiroki Meguro Amine Hallab Kevin Mihelc Jen Bacior Advisors: Kelly Dympna, MD John Patzer, PhD 22 March 2005

2. Project Overview Intended Use: Open Liver Biopsy Basic Functions 1). Tissue excision 2). Cauterization 3). Biopsy Tissue Protection

3. Personal Responsibilities Market Research Initial LEC Design Assist in Testing Overall Design History File Update

4. Personal Milestones Market Research: Done Initial LEC Blade Design : Done Assist in Prototype Testing: March 28 th – April 11 th Revise DHF : Week of April 4 th

5. Market Research 25 million people in America are affected by acute and chronic liver disease Liver biopsy is an essential tool in studying, categorizing and eventually curing liver disease There is an emerging need for a tool that will be useful when spot-probe freezing has been used prior to biopsy In 2001, alone, $262 million was spent on liver research

6. Initial LEC Blade Design Geometric approach 1. Triangle shape in order to excise the tissue as a wedge 2. Allows for a very sharp edge to prevent tissue damage

7. Initial LEC Design Blades

8. LEC Design Transition

9. Prototype Testing Determine criteria to adjudicate success of prototype –Accuracy of cut –Speed of cauterization –Final temperature of biopsy tissue –Ease of use

10. Overall Design History File Update Revise and Update file to confirm proper development of the LEC Biopsy Tool

11. Acknowledgements Professor Mark Gartner Kelly Dympna, MD John Patzer, PhD LEC Group Menbers Department of Bioengineering

12. Questions ??