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The Posterior Iliac Crest Outperforms the Anterior Iliac Crest When Obtaining Mesenchymal Stem Cells from Bone Marrow by Michela Pierini, Claudia Di Bella,

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Presentation on theme: "The Posterior Iliac Crest Outperforms the Anterior Iliac Crest When Obtaining Mesenchymal Stem Cells from Bone Marrow by Michela Pierini, Claudia Di Bella,"— Presentation transcript:

1 The Posterior Iliac Crest Outperforms the Anterior Iliac Crest When Obtaining Mesenchymal Stem Cells from Bone Marrow by Michela Pierini, Claudia Di Bella, Barbara Dozza, Tommaso Frisoni, Elisa Martella, Chiara Bellotti, Daniel Remondini, Enrico Lucarelli, Sandro Giannini, and Davide Donati J Bone Joint Surg Am Volume 95(12):1101-1107 June 19, 2013 ©2013 by The Journal of Bone and Joint Surgery, Inc.

2 Yield (Fig. 1-A) and viability (Fig. 1-B) of nucleated cells harvested from the bone marrow (n = 22 donors). Michela Pierini et al. J Bone Joint Surg Am 2013;95:1101- 1107 ©2013 by The Journal of Bone and Joint Surgery, Inc.

3 Yield (Fig. 2-A) and viability (Fig. 2-B) of mononuclear cells (MNCs) isolated from 16 mL of bone marrow by means of gradient separation (n = 22 donors). Michela Pierini et al. J Bone Joint Surg Am 2013;95:1101- 1107 ©2013 by The Journal of Bone and Joint Surgery, Inc.

4 Colony formation assays (n = 19 donors). Michela Pierini et al. J Bone Joint Surg Am 2013;95:1101- 1107 ©2013 by The Journal of Bone and Joint Surgery, Inc.

5 Osteogenic potential of mesenchymal stem cells (n = 17 donors, triplicate cultures). Michela Pierini et al. J Bone Joint Surg Am 2013;95:1101- 1107 ©2013 by The Journal of Bone and Joint Surgery, Inc.

6 Adipogenic potential of mesenchymal stem cells (n = 10 donors, triplicate cultures). Michela Pierini et al. J Bone Joint Surg Am 2013;95:1101- 1107 ©2013 by The Journal of Bone and Joint Surgery, Inc.

7 Chondrogenic potential of mesenchymal stem cells (n = 4 donors, duplicate cultures). Michela Pierini et al. J Bone Joint Surg Am 2013;95:1101- 1107 ©2013 by The Journal of Bone and Joint Surgery, Inc.


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