1. Diet Study for Nonalcoholic Fatty Liver Disease 022509 Lab meeting

3. Increased fatty acid uptake Increased de novo synthesis Reduced VLDL secretion Reduced fatty acid oxidation Steatosis

4. Diet Study for Steatosis High fat diet – non lipogenic diet Steatosis is likely to result from increased fatty acid uptake. High carbohydrate diet – lipogenic diet Steatosis is likely to result from de novo synthesis.

5. Diet Study for Steatosis Animals: Lean male B6 and Btbr, 5 animals each group Diet: Chow(15% fat from lard + 55% carbohydrate from sucrose) High fat diet (60% fat from lard + 10% carbohydrate from sucrose) High Carbohydrate diet (5% fat from lard + 70% carbohydrate from sucrose ) We will need approximately 5 kg per diet (~$50 per kg) Experimental design: Start diet from weaning, sacrifice at 10 week of age

6. Data collected Plasma at weaning, 6, 8, and 10 weeks (for glucose, TG and insulin measurement) Tissues collected at sacrifice: Liver (for TG and mRNA measurement) Adipose Pancreas Muscle Body weight at weaning, 6, 8, and 10 weeks