1. Write the complementary strand: 5’ T G A C A G C T T C 3’

2. Write the complementary strand: 5’ T G A C A G C T T C 3’ 3’ A C T G T C G A A G 5’

3.  DNA is made of subunits called nucleotides  DNA nucleotides are composed of a phosphate, deoxyribose sugar, and a nitrogen-containing base  The 4 bases in DNA are: adenine (A), thymine (T), guanine (G), and cytosine (C)  Located in the nucleus of the cell

4. Occurs when chromosomes duplicate and make exact copies of the DNA Occurs when chromosomes duplicate and make exact copies of the DNA Hydrogen bonds between bases are broken by helicase to “unzip” the molecule Hydrogen bonds between bases are broken by helicase to “unzip” the molecule Each old strand of nucleotides serves as a template for each new strand Each old strand of nucleotides serves as a template for each new strand New nucleotides move into complementary positions are joined by DNA polymerase New nucleotides move into complementary positions are joined by DNA polymerase

5. Modified version from Kim Useglia-former student teacher @ Lynn English Ms. Mezzetti Lynn English High School Science Department

6. If DNA is in the nucleus and enzymes and amino acid building blocks for proteins are in the cytosol how do the messages get sent through the cell?

7. The Central Dogma

8. 1.RNA has a sugar ribose DNA has a sugar deoxyribose 2.RNA contains the base uracil (U) DNA has thymine (T) 3.RNA molecule is single-stranded DNA is double-stranded

9. Messenger RNA (mRNA) carries genetic information to the ribosomes in the cytosol Ribosomal RNA (rRNA), along with protein, makes up the ribosomes Transfer RNA (tRNA) transfers amino acids to the ribosomes where proteins are synthesized

10.  Proteins are made of amino acids linked together by peptide bonds  20 different amino acids exist  Amino acids chains are called polypeptides  Segment of DNA that codes for the amino acid sequence in a protein are called genes

11.  Transcription makes an RNA molecule complementary to a portion of DNA  Translation occurs when the sequence of bases of mRNA DIRECTS the sequence of amino acids in a polypeptide

12.  DNA  RNA

13. 1) DNA unwinds and serves as a template for mRNA synthesis 2) RNA polymerase reads DNA and adds RNA nucleotides

14. Carries the information for a specific protein Made up of 500 to 1000 nucleotides long Single stranded

15. What would be the complementary RNA strand to following DNA sequence? 5’ G A T C G A T C A A C 3’

16. What would be the complementary RNA strand to following DNA sequence? 5’ G A T C G A T C A A C 3’ 3’ C U A G C U A G U U G 5’

17.  DNA contains a triplet code, Sequence of 3 bases called codon  Codes for amino acids  Every three bases on DNA stands for ONE amino acid  Each three-letter unit on mRNA is called a codon

18. DNA 5’ G A T C G A T C A A C G 3’ RNA 3’ C U A G C U A G U U G C 5’

19. DNA 5’ G A T C G A T C A A C G 3’ RNA 3’ C U A G C U A G U U G C 5’ CODONS: CUA GCU AGU UGC

20.  There are 20 amino acids with a possible 64 different triplets  Most amino acids have more than one codon!  The code is nearly universal among living organisms

23. AUG – methionine or start codon UAA, UAG, or UGA – stop codons

24. GGG UCA CAU GCA AAA

25. GGG Glycine UCA Tyrosine CAU Histodine GCA Alanine AAA Lysine

26. RNA  Protein

27. Translation is the process of decoding the mRNA into a polypeptide chain

28. Synthesis of proteins in the cytoplasm Involves the following: 1.mRNA (codons) 2.tRNA (anticodons) 3.ribosomes 4.amino acids

29. Made up of 75 to 80 nucleotides long Transports amino acids to the mRNA Have anticodons that are complementary to mRNA codons Recognizes the appropriate codons on the mRNA and bonds to them with H-bonds

30. amino acid attachment site UAC anticodon

31. Ribosomes read mRNA three bases or 1 codon (ex: GAC)at a time and construct the proteins Made up of rRNA and proteins

32. Made up of rRNA is 100 to 3000 nucleotides long Made inside the nucleus of a cell Associates with proteins to form ribosomes

33. Click to view Nova Protein Synthesis Video Click to view Nova Protein Synthesis Video

34. The end products of protein synthesis is a primary structure of a protein A sequence of amino acid bonded together by peptide bonds

36. DNAmRNA tRNA (ribosomes) Protein

37. Where does transcription take place? What is the role of mRNA? What is translation? Where does it take place? What is a codon? Anticodon? What is the role of tRNA? Ribosomes? How does the protein get made? Where does the protein go? What is it used for? Any questions?