1. 3.What is the principle behind the Quantiferon-Tb assay for TB?

2. What is the principle behind the Quantiferon-Tb assay for TB? Definition: It is a whole-blood test for use as an aid in diagnosing Mycobacterium tuberculosis infection – latent tuberculosis infection (LTBI) – tuberculosis (TB) disease

3. What is the principle behind the Quantiferon-Tb assay for TB? The principle relies on the quantitative (in vitro) release of interferon gamma by the lymphocytes, upon exposure of M. tuberculosis proteins antigens – include mixtures of synthetic peptides : ESAT-6 and CFP-10

4. Test tube 1 Nil Control tube Whole Blood Cell Incubation at 37°C for 16- 24hrs Interferon Gamma Measurements by ELISA Test Tube 3 Mitogen Control tube. Test tube 2 TB Antigen tube

5. Interpretation of Results