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Published byGarey Patrick Modified over 9 years ago
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Solutions & Dilutions Intro to Spectrophotometry
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SOLUTIONSDILUTIONS Solute Solvent Simple dilution Serial dilutions 1 can juice 4 cans water 1:5 dilution factor
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Serial Dilution Serial Dilution
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Spectrophotometry Light has: 1. A wave nature which gives rise to the visible spectrum of light 2. A particle nature which gives rise to the photoelectric effect (used in the spec.)
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Light Waves
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When Light Strikes Matter…
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The red end of the spectrum has been absorbed Blue light has been transmitted White Light Blue Light
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Color Wheel L (nm) Color Absorbed Color Seen 380-430VioletYellow 430-475BlueOrange 475-505 Green- Blue Red- Orange 505-555GreenRed 555-575 Yellow- Green Violet-Red 575-600YellowViolet 600-650OrangeBlue 650-780RedGreen
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Isolating Wavelengths
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Design of a Spectrophotometer
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Photoelectric Effect
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Light Transmission Dependence on Concentration Beer’s Law
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Cuvettes & The Blank
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Standardization Graph
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