1. Solutions & Dilutions Intro to Spectrophotometry

2. SOLUTIONSDILUTIONS Solute Solvent Simple dilution Serial dilutions 1 can juice 4 cans water 1:5 dilution factor

3. Serial Dilution Serial Dilution

4. Spectrophotometry Light has: 1. A wave nature which gives rise to the visible spectrum of light 2. A particle nature which gives rise to the photoelectric effect (used in the spec.)

5. Light Waves

6. When Light Strikes Matter…

7. The red end of the spectrum has been absorbed Blue light has been transmitted White Light Blue Light

8. Color Wheel L (nm) Color Absorbed Color Seen 380-430VioletYellow 430-475BlueOrange 475-505 Green- Blue Red- Orange 505-555GreenRed 555-575 Yellow- Green Violet-Red 575-600YellowViolet 600-650OrangeBlue 650-780RedGreen

9. Isolating Wavelengths

10. Design of a Spectrophotometer

11. Photoelectric Effect

12. Light Transmission Dependence on Concentration Beer’s Law

13. Cuvettes & The Blank

14. Standardization Graph