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Solutions & Dilutions Intro to Spectrophotometry.

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Presentation on theme: "Solutions & Dilutions Intro to Spectrophotometry."— Presentation transcript:

1 Solutions & Dilutions Intro to Spectrophotometry

2 SOLUTIONSDILUTIONS Solute Solvent Simple dilution Serial dilutions 1 can juice 4 cans water 1:5 dilution factor

3 Serial Dilution Serial Dilution

4 Spectrophotometry Light has: 1. A wave nature which gives rise to the visible spectrum of light 2. A particle nature which gives rise to the photoelectric effect (used in the spec.)

5 Light Waves

6 When Light Strikes Matter…

7 The red end of the spectrum has been absorbed Blue light has been transmitted White Light Blue Light

8 Color Wheel L (nm) Color Absorbed Color Seen 380-430VioletYellow 430-475BlueOrange 475-505 Green- Blue Red- Orange 505-555GreenRed 555-575 Yellow- Green Violet-Red 575-600YellowViolet 600-650OrangeBlue 650-780RedGreen

9 Isolating Wavelengths

10 Design of a Spectrophotometer

11 Photoelectric Effect

12 Light Transmission Dependence on Concentration Beer’s Law

13 Cuvettes & The Blank

14 Standardization Graph


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