1. Biotech Equations

2. Preparing Solutions What is a solution? – Mixture of two ore more substances Solutions have two parts – Solute – substance that is being dissolved – Solvent – substance doing the dissolving

3. Making Mass/Volume Solutions ____ g/mL X ____ mL = ____ g of solution concentrationvolumeto be weighed out, desireddesireddissolved in the solvent

4. What mass of the protein, gelatin, is needed to make 0.5 L of a 3 g/L gelatin solution?

5. Mass/Volume Concentration Equation ____ % = ____ percent valuedecimal value of the g/mL ____ X ____ = ____ g of solute to decimaltotal volumebe measured and valuedesired (mL)added to the volume desired of solvent

6. What mass of gelatin is needed to make 5 L of a 6% gelatin solution?

7. Molar mass of a substance – Find by using the periodic table! – The masses on the periodic table are of one mole of that substance – Mole – unit of measurement used to express the amount of a chemical substance

9. Find the molar mass of – NaCl – O 2 – HCl – CH 3 COOH

10. Find the molar mass of – NaCl  – O 2  – HCl  – CH 3 COOH  58.44 g/mol 32 g/mol 36.46 g/mol 60.06 g/mol

11. End for today

12. What if we are given grams of a substance…. Not moles?! If this happens we will have to do stoichiometry Going from grams of a substance to moles of that same substance How many moles will I have if I have 3 grams of Hydrogen?

13. Can also go the other way… I’m given moles and I need grams!! Convert to grams 2 mol sodium chloride

14. Molarity (M)

15. What is the molarity of a solution that contains 4.53 moles of lithium nitrate in 2.85 liters of solution?