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A B Figure S1 (related to Figure 1). BETi-tolerant A375 and NOMO-1 cells show an abrogated apoptotic response to BETi. C D E F.

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Presentation on theme: "A B Figure S1 (related to Figure 1). BETi-tolerant A375 and NOMO-1 cells show an abrogated apoptotic response to BETi. C D E F."— Presentation transcript:

1 A B Figure S1 (related to Figure 1). BETi-tolerant A375 and NOMO-1 cells show an abrogated apoptotic response to BETi. C D E F

2 A Log 2 FC -3 3 A375A375-t1A375-t2 CPI203 vs. DMSO Tolerant vs. Parental CPI203 NOMO-1 CPI203 CPI203 vs. DMSO Tolerant vs. Parental NOMO-1-t A375A375-t1A375-t2 CPI203 vs. DMSO NOMO-1 NOMO-1-t CPI203 vs. DMSO 1.0 5.7 5.2 A375 A375-t1 A375-t2 BCL2L1 GAPDH A375 A375-t1 A375-t2 LP-1 BCL2 GAPDH B C BCL2L1 BCL2 Vinculin Figure S2 (related to Figure 1). BETi-tolerant A375 and NOMO-1 cells show increased expression of BCL2 family genes.

3 A C D B E Clone 2 Clone 10 Vector BCL2L1  -actin F Figure S3 (related to Figure 2). Increased expression of BCL2 family members is important for the BETi tolerance phenotype.

4 A CD % depolarization, BAD peptide% depolarization, HRK peptide Cell linemeanSEMPmeanSEMPBCL2, RMABCL2L1, RMA MV41132.825.970.013.427.960.7110.664.68 Kasumi-131.057.020.01-12.096.790.238.654.62 NCI-H92935.386.430.0012.602.770.146.704.51 Daudi11.525.990.62-4.799.340.233.277.33 SUDHL-42.807.340.23-13.768.060.003.845.63 SUDHL-57.462.920.49-19.388.170.083.844.59 B Figure S4 (related to Figure 4). Basal expression of apoptotic factors is predictive of response to BETi.

5 p < 0.0001 p = 0.03p = 0.001 p = 0.06p = 0.02 A BCD p = 0.03 p < 0.0001 high low high low high low high low Figure S5 (related to Figure 4). Basal expression of apoptotic factors is predictive of response to BETi.

6 STD lowOPM2DOHH2K562Kasumi-1MOLM13RajiSUDHL4STD highTHP-1WSU-DLCL2SUDHL5LP1MV-411MC116REH BCL2L1 NamalwaMOLT4MOLT16ML2KMS26HL60Daudi CA46KARPAS422KMM1KMS27MOLP8H929PL21RPMI8226SET2U266 BCL2 cell line BCL2, ng/  g lysate BCL2L1, ng/  g lysateBCL2/BCL2L1 GI50,  M KARPAS4220.33190.00013366.180.90 KMS270.42820.00031480.670.14 DOHH21.31120.00131010.490.31 MOLM-130.69220.00071001.190.09 SUDHL40.93300.0027348.781.15 PL210.21620.0010215.780.11 Opm20.32400.0017189.810.08 MV4-110.33380.0019174.850.01 THP-10.23730.0020120.010.06 HL600.22260.002781.650.12 ML20.18090.0013136.900.15 Kasumi-10.31650.005161.990.02 REH0.52700.009655.020.39 WSU-DLCL20.30300.006248.560.22 MOLT160.15300.003543.940.00 Raji0.09880.002933.550.24 LP10.18490.006230.010.07 MOLP80.02130.000826.010.06 CA460.06020.002425.540.11 KMM10.16600.008619.290.15 RPMI82260.20830.012017.420.45 H9290.06710.004514.820.04 MC1160.17870.014512.361.60 U2660.22220.03576.231.18 KMS260.15430.03564.340.06 Namalwa0.02150.00603.580.11 SUDHL50.00170.00062.851.03 MOLT40.08490.03032.803.56 SET20.03070.01761.740.04 K5620.00840.03740.220.56 Daudi0.00280.02980.102.00 A B C STD lowSTD high STD lowSTD high STD lowSTD high STD low STD high Figure S6 (related to Figure 4). Basal expression of apoptotic factors is predictive of response to BETi.

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