1. Laboratory of bio cybernetics
Lea Retelj, Ajda Bicek, Matevz Leskovsek, Jasna Krmelj, Tadej Kotnik, Dusa Hodzic, Marko Usaj, Gorazd Pucihar, Alenka Macek Lebar, Samo Mahnic, Barbara Mali, Tomaz Jarm, Jaka Cemazar, Selma Corovic, Denis Pavliha, Bor Kos, Masa Kanduser, Natasa Pavselj, Janez Zigon, Ivan Pavlovic, Stanislav Rebersek, David Slavic, Melvin Essone, Jure Jelenc, Matej Rebersek, Tina Batista Napotnik, Mitja Gliha, Sasa Haberl, Peter Kramar, Igor Marjanovic, Barbara Zorec, Matej Kranjc, Damijan Miklavcic.
(Missing from the photo: Karel Flisar, Marija Marcan, Andraz Polak)

2. Applications of Electroporation
Exposure to
electric field
irreversible
Cell death
+ E
reversible
Introducing small
molecules
Extraction of
molecules
Introducing large
molecules EP
Cell fusion
Protein insertion in
the plasma membrane

3. ECT day 0
10 weeks after ECT
selectivity of ECT
Julie Gehl.
Ugeskrift for Laeger 167(34): , 2005.
Jarm, Cemazar, Miklavcic, Sersa.
Expert Rev. Anticancer Ther. 10: 729–746, 2010

4. Mode of operation in Laboratory of Biocybernetics

5. Capacities and experties
2 cell culture labs
1 cell culture lab safety level 2
1 microbiology cell lab
Skin ex vivo lab; lipid bilayer
Electronic workshop
Impedance and temperature measurements
Modelling (biological systems, electronic circuitry, multiphysics, multiscale…)
Cell electroporation
Lipid bilayer
Vesicles (GUV, SUV,…)
Cells (attached, suspension, in matrigel…)
Cells: single, clusteres
Non-excitable, excitable
Skin ex vivo
Extraction of intracellular components from cells
Dielectrophoresis for cell separation
Bioimpedance measurement; electrical characterisation

6. See also: www.electroporation.net
Damijan Miklavčič
University of Ljubljana
Faculty of Electrical Engineering
Ljubljana, SLOVENIA