1. The world of prokaryotes A- They’re everywhere 1- Collective prokaryote biomass outweighs all eukaryotes combined by at least tenfold. 2. They exist almost everywhere, including places where eukaryotes cannot. 3. Most prokaryotes are beneficial; we couldn’t live without them. (e.g. Nitrogen-fixing bacteria) 4. Some cause illness à bubonic plague, diphtheria, salmonella 5. Approximately 5000 species have been identified. Estimates of prokaryote diversity range from 400,000 to 4,000,000 species. Prokaryotes

2. . B. Bacteria and archaea are the two main branches of prokaryote evolution Archaea are thought to be more closely related to eukaryotes than to bacteria.

3. II. Structure, function, and reproduction of prokaryotes A. Most prokaryotes are unicellular. 1. Some species form aggregates of two or more individuals. B. Three (3) common shapes: cocci (round); bacilli (rod); helical (spiral) C. Prokaryotes are typically 1-5 μm in diameter, but some can be seen by the naked eye. - Eukaryotic cells are typically 10-100 μm in diameter.

4. D. Almost all prokaryotes have cell walls external to the plasma membrane. 1. Cell walls maintain cell shape. 2. Cell walls are composed of peptidoglycan. 3. There are two types of cell walls. Bacteria are grouped according to cell wall type.

5. a. Gram-positive bacteria have simple, thick cell walls. Their cell walls are composed of a relatively large amount of peptidoglycan. b. Gram-negative bacteria have less peptidoglycan and are more complex. They have a peptidoglycan layer surrounded by the plasma membrane and an outer membrane. - Gram-negative bacteria are typically more resistant to host immune defenses and antibiotics. Note that the two types of bacteria can be stained to determine which is gram-negative (pink) and gram-positive (purple) using a Gram Stain.

7. Peptidoglycan Gram Positive Gram Negative Plasma membrane Outer membrane Lipopolysaccharide layer

8. 4. Most prokaryotes secrete sticky substances that form a protective layer and enable them to adhere to substrates. a. The sticky protective layer secreted by prokaryotes is called the capsule. 5. Some prokaryotes adhere to substrates using pili. a. Some pili are specialized for DNA transfer. This process is called conjugation; note for later in class.

9. E. Many prokaryotes are motile - Some exceed speeds 100 times their body length per second. 1. Modes of movement – Note the three types: a. Flagellum - basal apparatus rotates the flagellum and propels the cell b. Corkscrew movement of spirochetes (helical) c. Some prokaryotes glide over jets of slimy secretions. 2. Many prokaryotes move toward or away from a stimulus = taxis. Chemotaxis is the movement toward or away from a chemical.

13. F. Cellular and genomic organization of prokaryotes is different from that of eukaryotes 1. Prokaryotes have no nucleus. 2. The nucleoid region in a prokaryotic cell consists of a concentrated mass of DNA. This mass of DNA is usually one thousand times less than what is found in a eukaryote. 3. A prokaryote may have a plasmid in addition to its major chromosome. A plasmid is a small ring of DNA that carries accessory genes. Usually these genes are for antibiotic resistance!

14. Asexual reproduction: Fission

15. G. Prokaryotes grow and adapt rapidly - The doubling time for E. coli is 20 minutes. Start with one E. coli cell. After 48 hours of doubling every 20 minutes, the mass of E. coli would be 10,000 times the mass of the earth. Bacteria do not have gene transfer by sexual reproduction, but do transfer genes. Why? This is an aid in adapting (evolving). 1. Three (3) ways for genes to be transferred between cells: a. Transformation – cell takes up genes from the surrounding environment. b. Conjugation – direct transfer of genes from one prokaryote to another. Use the sex pilus to conjugate. c. Transduction – viruses transfer genes between prokaryotes.

16. Prokaryotic conjugation

17. Bacterial transduction

18. 2. Endospores are resistant cells formed by some bacteria as a way to withstand harsh conditions. The cell replicates its chromosome and wraps it in a durable wall that can protect the chromosome from adverse conditions, e.g. boiling water, desiccation. When the environment is good again, the cell will revive to a new vegetative (growing) spore.

20. III. Nutritional and metabolic diversity A. All prokaryotes (and eukaryotes too) are grouped into four (4) categories according to how they obtain energy and carbon. 1. Photoautotrophs - Photosynthetic  use light as the energy source - CO 2 is the carbon source Example: Cyanobacteria; plants (eukaryotic).

21. 2. Chemoautotrophs - Energy from oxidation of inorganic substances (e.g. NH 4, and S) - CO 2 is the carbon source Example: Sulfolobus, Beggiatoa (shown on slide)

22. 3. Photoheterotrophs - Light as energy source - Organic compounds are source of carbon 4. Chemoheterotrophs - Organic compounds are energy source and source of carbon (this includes humans) Examples: Many prokaryotes; animals (eukaryotic); fungi (eukaryotic)

24. B. Metabolic relationships to oxygen 1. Obligate aerobes - Use O 2 for respiration; cannot grow without it. (Humans are obligate aerobes) 2. Facultative aerobes - Use O 2 when available; ferment when O 2 isn’t available. 3. Obligate anaerobes - Poisoned by O 2 ; use fermentation or live by anaerobic respiration. In anaerobic respiration, inorganic molecules like SO 4, NO 3, and Fe 3+ are used instead of oxygen.

25. C. Photosynthesis evolved early in prokaryotic life 1. Cyanobacteria started to produce O 2 about 2.7 billion years ago Contrasting hypotheses for the taxonomic distribution of photosynthesis among prokaryotes.

27. A. Great diversity of Archaea in extreme environments and oceans 1. Two taxa of archae: a. Euryarchaeota – most archae b. Crenarcheota – most thermophilic species 2. Examples of extremophiles a. Methanogens produce methane - Energy is from hydrogen gas - Strictly anaerobic - Inhabit swamps and animal intestines b. Extreme halophiles - Live in salty environments (Great Salt Lake)

28. c. Extreme thermophiles - 60- 80 °C optimum temperatures (hot springs) - 105 °C for deep-sea hydrothermal vents

29. V. Ecological impacts of prokaryotes A. Prokaryotes are links in the recycling of chemical elements B. Many prokaryotes are symbiotic (2 organisms living in direct contact with each other). There are three types of symbioses: 1. Mutualism – both symbiotic organisms benefit - e.g. Nitrogen-fixing bacteria like Rhizobium: plant obtain organic nitrogen, Rhizobium gets energy in the form of sugars that the plant produces. Another example:

30. Are all prokaryotes disease producing germs? Without prokaryotes ecosystems would collapse!

31. An overview of ecosystem dynamics

32. 2. Commensalism – one organism benefits and the other is not harmed. - e.g. Bacteria on our skin 3. Parasitism – parasite benefits and the host is harmed. C. Pathogens cause human diseases - Some pathogens are opportunistic. They may be normal residents of the host, but if the host is weakened, then they cause disease.

33. - How do we know if a particular organism is responsible for a disease? Robert Koch formed postulates as guidelines to establish that a disease is caused by a particular pathogen: a. Find same pathogen in each diseased individual. b. Isolate the pathogen and grow it in pure culture. c. Inoculate an individual with the isolated pathogen and the disease is induced. d. Isolate the same pathogen from the infected individual. This procedure is called Koch’s Postulates and is used widely to determine what infectious agent causes disease.

34. Most pathogens cause disease by producing poisons, these are either: - Exotoxins: proteins secreted by the pathogen that cause illness. -Endotoxins: poisons that are part of the pathogen that causes illness. (e.g. bacterium’s outer membrane) D. Humans use prokaryotes in research and technology Examples: Sewage treatment Bioremediation Chemical & Medical production Research (genetic engineering, etc.)