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Cell Count DNA Content 0 hr24 hr48 hr 72 hr Empty Vector Strep Claspin (Induced) Strep Claspin (Uninduced) DNA content (Dapi) Strep A B C McGarry et al.

Published byBelinda Paul Modified over 9 years ago

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Presentation on theme: "Cell Count DNA Content 0 hr24 hr48 hr 72 hr Empty Vector Strep Claspin (Induced) Strep Claspin (Uninduced) DNA content (Dapi) Strep A B C McGarry et al."— Presentation transcript:

1 Cell Count DNA Content 0 hr24 hr48 hr 72 hr Empty Vector Strep Claspin (Induced) Strep Claspin (Uninduced) DNA content (Dapi) Strep A B C McGarry et al. Fig S1

2 100mM 200mM 300mM400mM300mM400mM Soluble Insoluble Strep-Claspin Strep GAPDH H2A McGarry et al. Fig S2

3 siCTRL siUSP9X U2OS HEK293 Cell Count DNA Content (PI) 2N4N2N4N McGarry et al. Fig S3

4 McGarry et al. Fig S4

5 A B McGarry et al. Fig S5 0 9 6 3 Claspin GAPDH NT WT CD 0 9 6 3 0 9 6 3 Hours

6 siUSP9XγH2AXDapiMERGE siCTRL siClaspin siUSP9X (A) siUSP9X (B) 2mM HU 2 hr siCTRL siClaspin siUSP9X (A) siUSP9X (B) Phos CHK1 Ser-317 GAPDH Total CHK1 Claspin siCTRL siClaspin siUSP9X (A) siUSP9X (B) A B Claspin USP9X Total CHK1 GAPDH siCTRL siUSP9X 2mM HU 2 hr Phos CHK1 Ser-317 C McGarry et al. Fig S6

7 Untreated 6h LMB 8h LMB A B McGarry et al. Fig S7

8 Leptomycin treated Cell D Untreated Cell B Frontal Y slice (XZ view) Transversal X slice (YZ view) Axial Z slice (XY view) Maximum intensity projection on Z axis A B C Opaque nucleus Transparent nucleus No nucleus All USP9X objects Leptomycin treated Cell D Untreated Cell B McGarry et al. Fig S8

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